Understanding BLAST and Reverse Complementary RNA, and Discussing miRcore’s Future Leadership

0 24 March 2018

3/24/2018 – Blog Post

Eleanor Lin

During the March 24th meeting, members were introduced to BLAST, the Basic Local Alignment Search Tool (https://blast.ncbi.nlm.nih.gov/Blast.cgi). The focus was on using Nucleotide BLAST to match RNA sequences from a file type called FASTA to RNA sequences in the NCBI database. FASTA files contain a name for each sequence and the order of the nucleotides in the sequence. Lines from a FASTA file can be copy-pasted into BLAST, which will provide a visual summary of the matches that it has found for the input sequence. It also lists out information for each individual sequence match.UntitledDuring the meeting, the concept of reverse complementary DNA sequences was reviewed, in order to explain why BLAST will label some sequence matches as “Plus/Plus” and others as “Plus/Minus.” DNA

Posted in 2017-18 School Year by Srikar Munukutla
0 17 March 2018

A Summary of Dr. Castro’s Talk (3/10/18) Prasanna Padmanabham


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Last week on 3/10/18, Dr. Castro presented her research on treating Lower Grade Gliomas by injecting a vector that allowed immune cells to identify and kill tumor cells.   Her treatment hypothesis solves a big problem doctors have had with brain cancer. Since tumors grow in the brain, doctors must remove enough tissue to get rid of the cancer cells, but be careful they don’t remove too much as that can impact the person’s life. This is a big problem in children because their brains are still developing and removing too much tissue can cause developmental issues. Patients that undergo extensive surgery tend to live the longest, but they too still have a very big risk of another tumor growing again.   In Dr. Castro’s model, immune cells are trained to kill cancer cells and this prevents the tumor from growing again. The have conducted many models in rats, and the rats that receive the treatmen

0 15 March 2018

This year’s disease of focus is Brain Cancer.

Posted in 2017-18 School Year by mircore